rabbit anti cd44 fitc  (Bioss)

Journal: International journal of medical sciences

Article Title: Atranorin driven by nano materials SPION lead to ferroptosis of gastric cancer stem cells by weakening the mRNA 5-hydroxymethylcytidine modification of the Xc-/GPX4 axis and its expression.

doi: 10.7150/ijms.73701

Figure Lengend Snippet: Figure 1. Atranorin@SPION inhibit the in vitro activity of CD44+/CD24+ GCSCs. (A) Molecular structure of Atranorin. (B) MTT results showing that Atranorin@SPION significantly inhibited the proliferation of GCSCs in vitro. **p < 0.01 vs. SPION; t test; n = 4. (C) GCSC phenotype in each group under the light microscope. The magnification was 200 ×. (D) The results of flow cytometry showing that Atranorin@SPION significantly induced GCSC apoptosis in vitro. **p < 0.01 vs. SPION; t test; n = 4. (E) Transwell assay showing that Atranorin@SPION significantly inhibited the migration of GCSCs in vitro. **p < 0.01 vs. SPION; t test; n = 4. (F) Atranorin@SPION inhibit the angiogenesis phenotype in Zebrafish. (G) Atranorin@SPION inhibit angiogenesis in Zebrafish. **p < 0.01 vs. SPION; t test; n = 4.

Article Snippet: The volume of the cell suspension was adjusted and 4 μL of fluorescein isothiocyanate (FITC)-labelled rabbit anti-human CD44 monoclonal antibody and Cy3-labelled rabbit anti‐human CD24+ antibody (eBioscience, San Diego, CA, USA) were added to 100 μL of cell suspension and incubated in the dark at 4 °C for 30 minutes.

Techniques: In Vitro, Activity Assay, Light Microscopy, Flow Cytometry, Transwell Assay, Migration

Journal: International journal of medical sciences

Article Title: Atranorin driven by nano materials SPION lead to ferroptosis of gastric cancer stem cells by weakening the mRNA 5-hydroxymethylcytidine modification of the Xc-/GPX4 axis and its expression.

doi: 10.7150/ijms.73701

Figure Lengend Snippet: Figure 2. Atranorin@SPION promoted the accumulation of lipid peroxides and inhibited the expression of ferroptosis-related protective proteins in CD44+/ CD24+ GCSCs. (A) qRT-PCR results showing that Atranorin@SPION significantly downregulated the mRNA expression of stem cell markers and ferroptosis protective genes in GCSCs. **p < 0.01 vs. SPION; *p < 0.05 vs. SPION; t test; n = 4. (B) The results of biochemical detection showing that Atranorin@SPION significantly downregulated the concentration of lactic acid and anti-superoxide anions in GCSCs, and significantly upregulated extent of LPO and the concentration of Fe2+. **p < 0.01 vs. SPION; *p < 0.05 vs. SPION; t test; n = 4. (C) Western blotting results showing that Atranorin@SPION significantly downregulated the levels of ferroptosis-related protective proteins in GCSCs.

Article Snippet: The volume of the cell suspension was adjusted and 4 μL of fluorescein isothiocyanate (FITC)-labelled rabbit anti-human CD44 monoclonal antibody and Cy3-labelled rabbit anti‐human CD24+ antibody (eBioscience, San Diego, CA, USA) were added to 100 μL of cell suspension and incubated in the dark at 4 °C for 30 minutes.

Techniques: Expressing, Quantitative RT-PCR, Concentration Assay, Western Blot

Journal: International journal of medical sciences

Article Title: Atranorin driven by nano materials SPION lead to ferroptosis of gastric cancer stem cells by weakening the mRNA 5-hydroxymethylcytidine modification of the Xc-/GPX4 axis and its expression.

doi: 10.7150/ijms.73701

Figure Lengend Snippet: Figure 3. Atranorin@SPION significantly downregulated the mRNA hm5C modification level of CD44+/CD24 +GCSCs. (A) HPLC-MS results showing that there were significant differences in multiple mRNA modifications between the two groups. (B) Atranorin@SPION significantly downregulated the mRNA levels of hm5C, ncm5U, and m2G in GCSCs. (C) Dot blot results showing that Atranorin@SPION significantly downregulated the hm5C modification of GCSCs mRNA. *p < 0.05 vs. SPION; t test; n = 4.

Article Snippet: The volume of the cell suspension was adjusted and 4 μL of fluorescein isothiocyanate (FITC)-labelled rabbit anti-human CD44 monoclonal antibody and Cy3-labelled rabbit anti‐human CD24+ antibody (eBioscience, San Diego, CA, USA) were added to 100 μL of cell suspension and incubated in the dark at 4 °C for 30 minutes.

Techniques: Modification, Dot Blot

Journal: International journal of medical sciences

Article Title: Atranorin driven by nano materials SPION lead to ferroptosis of gastric cancer stem cells by weakening the mRNA 5-hydroxymethylcytidine modification of the Xc-/GPX4 axis and its expression.

doi: 10.7150/ijms.73701

Figure Lengend Snippet: Figure 5. Atranorin@SPION significantly weakened the tumorigenicity of CD44+/CD24+ GCSCs in immunodeficient mice. (A) Morphology of dorsal tumors in the tumor-bearing mice. (B) Nuclear magnetic resonance imaging of tumors in the tumor-bearing mice. (C) The Atranorin@SPION group had a smaller tumor volume and weight than the control group. *p < 0.05 vs. SPION; t test; n = 4. (D) H & E staining confirming that the tumor tissues in each group were gastric cancer samples. (E) Immunohistochemical staining results showing significantly decreased expression levels of GPX4, SLC7A11, KI67, and TET1 in the Atranorin@SPION group were.

Article Snippet: The volume of the cell suspension was adjusted and 4 μL of fluorescein isothiocyanate (FITC)-labelled rabbit anti-human CD44 monoclonal antibody and Cy3-labelled rabbit anti‐human CD24+ antibody (eBioscience, San Diego, CA, USA) were added to 100 μL of cell suspension and incubated in the dark at 4 °C for 30 minutes.

Techniques: Nuclear Magnetic Resonance, Imaging, Control, Staining, Immunohistochemical staining, Expressing